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Flow cytometry measurements of human bone marrow CD34+ cells stably expressing ZBTB7A WT or mutants are shown. a Representative results of cells primed for differentiation into erythrocytes using <t>StemSpan</t> Erythroid Expansion Supplement for 7 days. b Summary of three independent experiments. c Representative results of cells primed for differentiation into monocytes and granulocytes using HemaTox Myeloid Kit for 7 days. d Summary of three independent experiments. e Schematic representation of the effects of ZBTB7A and RUNX1–RUNX1T1 expression on hematopoietic linage commitment.
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Flow cytometry measurements of human bone marrow CD34+ cells stably expressing ZBTB7A WT or mutants are shown. a Representative results of cells primed for differentiation into erythrocytes using <t>StemSpan</t> Erythroid Expansion Supplement for 7 days. b Summary of three independent experiments. c Representative results of cells primed for differentiation into monocytes and granulocytes using HemaTox Myeloid Kit for 7 days. d Summary of three independent experiments. e Schematic representation of the effects of ZBTB7A and RUNX1–RUNX1T1 expression on hematopoietic linage commitment.
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Flow cytometry measurements of human bone marrow CD34+ cells stably expressing ZBTB7A WT or mutants are shown. a Representative results of cells primed for differentiation into erythrocytes using <t>StemSpan</t> Erythroid Expansion Supplement for 7 days. b Summary of three independent experiments. c Representative results of cells primed for differentiation into monocytes and granulocytes using HemaTox Myeloid Kit for 7 days. d Summary of three independent experiments. e Schematic representation of the effects of ZBTB7A and RUNX1–RUNX1T1 expression on hematopoietic linage commitment.
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Flow cytometry measurements of human bone marrow CD34+ cells stably expressing ZBTB7A WT or mutants are shown. a Representative results of cells primed for differentiation into erythrocytes using <t>StemSpan</t> Erythroid Expansion Supplement for 7 days. b Summary of three independent experiments. c Representative results of cells primed for differentiation into monocytes and granulocytes using HemaTox Myeloid Kit for 7 days. d Summary of three independent experiments. e Schematic representation of the effects of ZBTB7A and RUNX1–RUNX1T1 expression on hematopoietic linage commitment.
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Flow cytometry measurements of human bone marrow CD34+ cells stably expressing ZBTB7A WT or mutants are shown. a Representative results of cells primed for differentiation into erythrocytes using <t>StemSpan</t> Erythroid Expansion Supplement for 7 days. b Summary of three independent experiments. c Representative results of cells primed for differentiation into monocytes and granulocytes using HemaTox Myeloid Kit for 7 days. d Summary of three independent experiments. e Schematic representation of the effects of ZBTB7A and RUNX1–RUNX1T1 expression on hematopoietic linage commitment.
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Flow cytometry measurements of human bone marrow CD34+ cells stably expressing ZBTB7A WT or mutants are shown. a Representative results of cells primed for differentiation into erythrocytes using <t>StemSpan</t> Erythroid Expansion Supplement for 7 days. b Summary of three independent experiments. c Representative results of cells primed for differentiation into monocytes and granulocytes using HemaTox Myeloid Kit for 7 days. d Summary of three independent experiments. e Schematic representation of the effects of ZBTB7A and RUNX1–RUNX1T1 expression on hematopoietic linage commitment.
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STEMCELL Technologies Inc stemspan serum-free expansion motif (sfem) ii
Flow cytometry measurements of human bone marrow CD34+ cells stably expressing ZBTB7A WT or mutants are shown. a Representative results of cells primed for differentiation into erythrocytes using <t>StemSpan</t> Erythroid Expansion Supplement for 7 days. b Summary of three independent experiments. c Representative results of cells primed for differentiation into monocytes and granulocytes using HemaTox Myeloid Kit for 7 days. d Summary of three independent experiments. e Schematic representation of the effects of ZBTB7A and RUNX1–RUNX1T1 expression on hematopoietic linage commitment.
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Flow cytometry measurements of human bone marrow CD34+ cells stably expressing ZBTB7A WT or mutants are shown. a Representative results of cells primed for differentiation into erythrocytes using StemSpan Erythroid Expansion Supplement for 7 days. b Summary of three independent experiments. c Representative results of cells primed for differentiation into monocytes and granulocytes using HemaTox Myeloid Kit for 7 days. d Summary of three independent experiments. e Schematic representation of the effects of ZBTB7A and RUNX1–RUNX1T1 expression on hematopoietic linage commitment.

Journal: Oncogene

Article Title: ZBTB7A prevents RUNX1-RUNX1T1-dependent clonal expansion of human hematopoietic stem and progenitor cells

doi: 10.1038/s41388-020-1209-4

Figure Lengend Snippet: Flow cytometry measurements of human bone marrow CD34+ cells stably expressing ZBTB7A WT or mutants are shown. a Representative results of cells primed for differentiation into erythrocytes using StemSpan Erythroid Expansion Supplement for 7 days. b Summary of three independent experiments. c Representative results of cells primed for differentiation into monocytes and granulocytes using HemaTox Myeloid Kit for 7 days. d Summary of three independent experiments. e Schematic representation of the effects of ZBTB7A and RUNX1–RUNX1T1 expression on hematopoietic linage commitment.

Article Snippet: A total of 5000 Human CD34+ bone marrow cells were seeded either in StemSpan SFEM with StemSpan Erythroid Expansion Supplement or HemaTox Myeloid Kit (StemCell Technologies) in a 96-well plate.

Techniques: Flow Cytometry, Stable Transfection, Expressing